10 selections by the Ottawa Senators in the 2000 NHL Entry Draft.
| Year | Pick | Round | Team | Player | Pos | Amateur Club | League | Country |
|---|---|---|---|---|---|---|---|---|
| 2000 | 21 | 1 | Ottawa Senators | Anton Volchenkov | D | CSKA 2 | RPL | RUS |
| 2000 | 45 | 2 | Ottawa Senators | Mathieu Chouinard | G | Shawinigan | QMJHL | CAN |
| 2000 | 55 | 2 | Ottawa Senators | Antoine Vermette | C | Victoriaville | QMJHL | CAN |
| 2000 | 87 | 3 | Ottawa Senators | Jan Bohac | C | Slavia Praha | CZECH | CZE |
| 2000 | 122 | 4 | Ottawa Senators | Derrick Byfuglien | D | Fargo-Moorhead | — | USA |
| 2000 | 156 | 5 | Ottawa Senators | Greg Zanon | D | U. of Nebraska-Omaha | CCHA | CAN |
| 2000 | 157 | 5 | Ottawa Senators | Grant Potulny | F | Lincoln | USHSE | USA |
| 2000 | 158 | 5 | Ottawa Senators | Sean Connolly | D | Northern Michigan U. | CCHA | USA |
| 2000 | 188 | 6 | Ottawa Senators | Jason Maleyko | D | Brampton | OHL | CAN |
| 2000 | 283 | 9 | Ottawa Senators | James Demone | D | Portland | WHL | CAN |