10 selections by the Tampa Bay Lightning in the 2000 NHL Entry Draft.
| Year | Pick | Round | Team | Player | Pos | Amateur Club | League | Country |
|---|---|---|---|---|---|---|---|---|
| 2000 | 8 | 1 | Tampa Bay Lightning | Nikita Alexeev | RW | Erie | OHL | RUS |
| 2000 | 34 | 2 | Tampa Bay Lightning | Ruslan Zainullin | RW | Ak-Bars Kazan | Russia U18 | RUS |
| 2000 | 81 | 3 | Tampa Bay Lightning | Alexandre Kharitonov | RW | Dynamo Moscow | KHL | RUS |
| 2000 | 126 | 4 | Tampa Bay Lightning | Johan Hagglund | C | Modo Jr. | Sweden | SWE |
| 2000 | 161 | 5 | Tampa Bay Lightning | Pavel Sedov | RW | Voskresensk | — | RUS |
| 2000 | 191 | 6 | Tampa Bay Lightning | Aaron Gionet | D | Kamloops | WHL | CAN |
| 2000 | 222 | 7 | Tampa Bay Lightning | Marek Priechodsky | D | Bratislava | SVK | SVK |
| 2000 | 226 | 7 | Tampa Bay Lightning | Brian Eklund | G | Brown U. | ECAC | USA |
| 2000 | 233 | 8 | Tampa Bay Lightning | Alexander Polukeyev | G | St. Petersburg Ska St. Petersburg | RUS | RUS |
| 2000 | 263 | 9 | Tampa Bay Lightning | Thomas Ziegler | F | HC Ambri Piotta | — | CHE |